Chromosome analysis of five longterm cell culture populations derived from non-leukemic human peripheral blood (Detroit strains).

The cultivation of adult human cells of normal origin has become a routine procedure during the past several years (4). Among the first stable epithelial-like (Ep-L) cell lines derived from nor mal human tissues were those established by Chang (1954) (5) from conjunctiva, liver, kidney, and appendix. Although the HeLa strain of cells from cancer tissue had been available before this time, there were objections to the use of cell strains derived from cancers for the cultivation of viruses for vaccine production, or in experi ments designed to explain the normal biology of human cells. The development of stable cell lines1 from normal tissue was at first thought to